The BioFilm Ring Test ® has been thoroughly validated
The proof of concept has been conducted in collaboration with a group of INRA (the French national research institute for agronomic research) and have been published in a peer-reviewed journal: Chavant et al., Journal of Microbiological Methods (2007) 68, 605-12.
For instance, scanning electron microscopy clearly shows that the beads are immobilized by the biofilm matrix of Listeria monocytogenes (Fig. 1) and Staphylococcus xylosus (Fig. 2), which are respectively low and high EPS producers.
The technology has already been applied on a wide variety of bacterial and algal micro-organisms:
- Escherichia coli
- Staphylococcus
- Pseudomonas
- Lactobacillus
- Salmonella
- Streptococcus
- Arthrospira
- Rhodella
- Listeria
- …
The BioFilm Ring Test ® holds many advantages over the Cristal Violet method:


